Articles

Tissue Culture

➢ Tissue Culture:

✓ The general term for the removal of cells, tissues or organs from an animal or plant and their subsequent placement into an artificial environment conducive to growth.

• Can be used to prepare finite or continuous cell cultures

• Will be similar biochemically and physiologically to parent tissue

Organ Culture

• Organ Culture

– The culture of whole organs or intact organ fragments with the intent of studying their continued function or development.

» Can be maintained for hours or days by perfusion with oxygenated blood or serum.

» Used for metabolism and drug studies

» Provides most accurate reflection of organism’s physiology

Organ Culture

• Organ Culture

» heart perfusion w/stem cells created a “new heart”.

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Cell Culture

➢ Cell Culture

✓ When cells are removed from the organ fragments prior to, or during cultivation, thus disrupting their normal relationships with neighboring cells.

• General term explaining non-”in vivo” experiments

• Non-tissue growth of plant and animal cells

Cell Culture

➢ Cell Culture

✓ When cells are removed from the organ fragments prior to, or during cultivation, thus disrupting their normal relationships with neighboring cells.

• General term explaining non-”in vivo” experiments

• Non-tissue growth of plant and animal cells

Mammalian Cell Culture

➢ Mammalian Cell Culture

✓ Cell culture of mammalian cells.

➢ Eukaryotic cells are much more difficult to culture than most prokaryotes.

➢ They demand complex media

➢ They are very susceptible to contamination and overgrowth by microbes such as bacteria, yeasts and fungi.

Cell Culture

➢ Two types of cell...