Submitted by: Submitted by organicme
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Words: 971
Pages: 4
Category: Science and Technology
Date Submitted: 11/02/2011 09:19 AM
TLC OF ANALGESIS DRUGS
Introduction:
This was an experiment of a thin-layer chromatography (TLC) used to determine the composition of various over-the-counter analgesics. Chromatography is a technique used to separate and identify individual components in a mixture. Adsorption chromatography was a technique makes used of the fact that components of a mixture tended to move at different speeds due to the differing attractions of the components to the coating material compared to the tendency of the components to remain in the moving fluid. During the elution with the solvent, the sample was partition itself between the stationary phase -the adsorbent layer; and the moving phase- the solvent so that the distance which the sample moves up the plate was characteristic of that substance and would differ from one substance to the next. Separation of a mixture into the component parts was achieved by elution with solvents of different polarity. The principles of polarity and of intermolecular attractions were of importance in determining how compounds would behave during the separation. While the more polar components of a mixture would stick more strongly to the polar stationary phase and thus, move slower, the least polar components would move the fastest and run higher up the plate. A mixture of 95% ethyl acetate and 5% acetic acid was used as the solvent for the chromatogram. The best known of substance that relieves pain is aspirin, but several other chemically similar compounds were also used as analgesics. The reference compounds were used: Caffeine, aspirin, ibuprofen and NoDoZ... Caffeine is sometime added to these formulations to overcome drowsiness. For cold remedies and decongestants aspirin and caffeine are primary ingredients. In addition to the active ingredients, the tablets of these drugs contain starch, lactose, and other substances that act as binders and permit rapid solution, and sometimes also inorganic bases.
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