Enzyme Lab

Title: Enzyme Lab

Introduction:

In this lab report I will be explaining what pH is , what an Enzyme is and does, what a chemical reaction is, and why liver was used in the experiment. An Enzyme is any of various proteins, originating from living cells and capable of producing certain chemical changes in organic substances by catalytic action. Catalase is an enzyme, which is found in many cells, but in highest level of liver because liver often functions to break down toxins found in the blood. Catalase catalyzes the break down of Hydrogen Peroxide.

The combination of two Hydrogen atoms with one Oxygen atom is PH which forms water. The PH is a measure of how acidic or basic solution is. The reason that liver was involved in this experiment was because the liver is a tissue that measures various enzymes released by the liver into the blood, and other living functions. Liver enzymes may be elevated when damage occurs in the liver leaving the organism to be sick and weak.

Hypothesis:

Liver placed in HCI or NaOH will develop a white foamy effect.

Materials:

(list of all materials used)

• !molar HCI solution (in dropper bottle)

• 1molar NaOH solution (in dropper bottle)

• 6 Test tubes and Test tube holder

• 10-ml Graduated cylinder

• 40 ml 3% Hydrogen peroxide solution (found in stores)

• Scissors and Forceps (tweezers)

• Thermometer

• Stirring rod

• PH paper

• Fresh liver

Procedure:

(steps in the order they were performed)

1. Placed 2 ml of the 3% hydrogen peroxide solution in the test tube.

2. Used forceps to add a small puce of liver and added it to the test tube, and observed the bubbles.

3. Poured off the liquid from test tube 1 into a clean test tube labeled (#2).

4. Tested the reaction by adding another piece of liver to the test tube (#2) and recorded the reaction rate in data table.

5. Added another 2 ml of hydrogen peroxide to the liver remaining in test tube #1 and recorded the reaction rate in...