Uplc

Shutting down the system

Caution: Buffers left in the detector can precipitate and damage instrument components. Tips: • If you are using Empower software to control the system, set system shutdown parameters in the Instrument Method. Consult the Empower online Help for more information. If you are using MassLynx software to control your system, set system shutdown parameters in the Shutdown Editor. Consult the MassLynx online Help for more information.

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Shutting down for less than 24 hours

To shut down the system for less than 24 hours 1. Continue to pump the initial mobile phase mixture through the column. Doing so maintains the column equilibrium necessary for good retention time reproducibility. To lengthen lamp life, extinguish the detector lamp by clicking (Lamp Off) in the detector control panel. Tip: If you are running under MassLynx control, ensure that Auto-Shutdown for your shutdown method is deactivated. 3. If a few hours will pass before the next injection, slow the flow rate in the interim to a few tenths of a mL/min to conserve solvent. Tip: Ensure that the shutdown method is deactivated. 4. Keep the detector operating and the column heater at operating temperature during this period.

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Shutting down the system

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Shutting down for more than 24 hours

To shut down the system for more than 24 hours 1. 2. 3. To lengthen lamp life, extinguish the detector lamp by clicking (Lamp Off) in the detector control panel. Remove buffer salts and additives by flushing with water. Flush the column and flow cell with 100% pure organic solvent. See also: Waters ACQUITY UPLC BEH Column Care and Use Instructions or ACQUITY UPLC HSS Column Care and Use Instructions. Warning: Risk of electric shock. The power switch on each system instrument controls the basic operational state of that instrument. Nevertheless, some instrument circuits remain live after the instrument is switched off. To completely interrupt power to a system instrument, set the power switch to...