Spectrophotometer

BACHELOR IN SCIENCE PHARMACEUTICAL CHEMISTRY (HONS)

SEMESTER 1

BIOLOGICAL SCIENCE

PRACTICAL REPORT 1

TITLE: SPECTROPHOTOMETER AND ITS FUNCTION

LECTURER : DR.HO KET LI

NAME: SYARIFAH ANITH ADILA BINTI SYED ROZHAN

STUDENT ID:00000011352

COHORT :PC1/14

BENCH 2 , MDL 3.03.05

Table of content

Content | Page |

Introduction | |

Objectives | |

Materials | |

Part 1 : Determination of Amax of Bromophenol Blue * Method * Results * Table1.1 * Graph 1 * Discussion | |

Part 2 : The Effect of Concentration on Absorbance of Bromophenol Blue Solution * Method * Results * Table 1.2a * Graph 2 * Discussion | |

Part 3 : Determination of The Concentrations of The Bromophenol Blue Solutions of Unknown Concentration * Method * Calculation * Discussion | |

Part 4 : The Effect of Concentration on Absorbance of Methyl Orange Solutions * Method * Results * Table 1.2b * Graph 3 * Discussion | |

Part 5: Determination of The Concentrations of Two Different Solutes, Bromophenol Blue and Methyl Orange , in Mixture C * Method * Results * Table 1.3 * Table 1.4 * Calculation * Discussion | |

Conclusion | |

References | |

Introduction

The uses of spectrophotometer has been a huge contribution in finding the concentration of solute or compound in chemical and biological sciences [1]. It is an instrument where it measure the concentration of solute or compound by measuring the amount of light absorbed by a sample [2]. Every compound or solute can be determined using the wavelength from the spectrophotometer. Usually different compounds or solute can absorbed light at a different wavelength [1]. The sample that was measured is placed in a cuvette. Cuvette is a small square tube cell that is made of plastic, glass or an optical grade quartz that was designed to hold a sample [3]. The cuvette is a very delicate and it should be handle with care because it can affect the...