Spectrophotometer and Its Functions

Table of contents

Page no.

Title 1

Table of contents 2

Objectives 3

Introduction 4

Materials and Methods 5-6

Results 7-10

Discussion 11

Conclusion 12

References 13

Objectives

1 . To determine the wavelength of maximum absorption, Amax , of Bromophenol blue.

2. To construct a standard concentration curve for Bromophenol blue

3. To determine the concentration of the unknown Bromophenol solutions.

4. To determine the concentration of two different solutes, Bromophenol blue and methyl orange,

in a mixture.

Introduction

A Spectrophotometer was an instrument used to measure the absorbance of a certain solution towards light. It analyzes and measures the amount and intensity of light absorbed that was passed through a solution. (2)

The basic function of a Spectrophotometer was to take in light, break it into spectral components also known as grating, and the grating was rotated to ensure the desired wavelength reaches the exit slit and passes through the solution in the cuvette. Then, the detector detects the amount of photons absorbed by the solution and displays its reading on the screen. The spectrophotometer can be separated into 2 parts which are spectrometer and photometer. The spectrometer conswasts a prwasm or grating that splits the light beam into several wavelengths and a slit that transmits the desired wavelength to the cuvette. The photometer conswasts a detector that detects the amount of light absorbed and sends readings to the display. (1)

To use a spectrophotometer correctly, the spectrophotometer has to be fwast re-zero or calibrated before every reading for the desired wavelength. A blank solution usually distilled water was added into the cuvette and then inserted into the compartment in the spectrophotometer. The cuvette has to be cleaned with paper towel or twassue to ensure no fingerprints or dust sticks on the surface of the cuvette to ensure a...