Submitted by: Submitted by br5200yi
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Category: Science and Technology
Date Submitted: 02/25/2016 08:26 AM
LABORATORY
Agar – the substance that causes substance to remain solid
PDA Potato Dextrose Agar – “selective” for fungus (yeasts, molds)
Photosynthesis – takes in CO2, puts out O2
Protozoa – unicellular heterotrophic eukaryotes, lack cell wall, classified by motility
Algae – photosynthetic eukaryotes characterized by chloroplasts and absence of roots. Produce 75% of Earth’s O2 by photosynthesis
MSA plates contain a high amount of salt to select for STAPHYLOCCUCUS.
Mouth epithelial cells revealed most bacteria were coccus shaped and associated with our cells
Hemolysis- B (yellow, very revealed) A (green, somewhat revealed) Y (hard to see) our mouth had some A and a little Y
B completely destroy RBCs, A partially destroy the RBCs, Y does not destroy RBCs.
MSA plates are high in salt that select for staphylococcus
Pathogenic staphylococcus ferment mannitol plates to yellow due to acid production
Gram-stain – FIRST test performed to identify bacteria, goalis to have single, well-isolated colonies.
Gram-pos turn out violent, gram-neg turn pink AFTER safranin
Gram-pos have the peptidoglycan very vulnerable
Order of Gram-stain: Fixation, Crystal Violet, Iodine, Decolorization, Safranin
Molds are multicellular fungi. Starts with formation as SPORE forms. Branches out, branches called HYPHAE. Collectively called MYCELIUM. VEGETATIVE HYPHAE absorb nutrition from the environment.
TEMPERATURE ORGANISMS
Psychrophiles - -10 to 20C
Psychrotrophs – 0 to 30C
Mesophiles – 10 to 50 ( MOST COMMON)
Thermophiles – 40 to 70C
Hyperthermophiles – 65 to 110C
Many human pathogens grow at 37C.
Systemic exposure – full body exposure
LECTURE
Likelihood of infection - (Number of Microbes)(Virulence)/(Host Immunity)
Hooke saw the first microbe
Leeuwenhoek developed the microscope, saw “animalcules”
Pasteur founded pasteurization, immunology and vaccination, germ theory
Bacteria and Archaea were around 1 billion years before photosynthetic...