Gel Electrophoresis
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Category: Science and Technology
Date Submitted: 06/16/2016 10:11 AM
The Biotechnology Education Company®
®
101
EDVO-Kit #
Principles and
Practice of Agarose
Gel Electrophoresis
Storage: Store the entire experiment
at room temperature
EXPERIMENT OBJECTIVE:
The objective of this experiment is to develop a
basic understanding of electrophoretic theory,
and gain “hands-on” familiarity with the procedures involved in agarose gel electrophoresis to
separate biological molecules.
All components are intended for educational research only.
They are not to be used for diagnostic or drug purposes, nor
administered to or consumed by humans or animals.
The Biotechnology Education Company ® • 1-800-EDVOTEK • www.edvotek.com
EVT 008114K
2
EDVO-Kit # 101
Principles and Practice of Agarose Gel Electrophoresis
Table of Contents
Page
Experiment Components
Experiment Requirements
3
3
Background Information
Agarose Gel Electrophoresis
About Electrophoresis Equipment
4
8
Experiment Procedures
Experiment Overview
Agarose Gel Preparation
Sample Delivery (Gel Loading)
Conducting Agarose Gel Electrophoresis
Experiment Results and Study Questions
12
13
17
19
22
Instructor's Guidelines
Notes to the Instructor
Pre-Lab Preparations
Batch Agarose Gel Preparation
Experiment Results and Analysis
Study Questions and Answers
23
25
28
29
30
Material Safety Data Sheets
31
EDVOTEK, The Biotechnology Education Company are registered trademarks of
EDVOTEK, Inc. UltraSpec-Agarose is a trademark of EDVOTEK, Inc.
EVT 008114K
The Biotechnology Education Company ® • 1-800-EDVOTEK • www.edvotek.com
EDVO-Kit # 101
Principles and Practice of Agarose Gel Electrophoresis
Experiment Components
ELECTROPHORESIS SAMPLES
•
Ready-to-Load™ Dye samples
A Orange
B Purple
C Red
D Blue 1
E Dye Mixture
F
Blue Dye Mixture (Blue 1 + Blue 2)
Storage:
Store entire experiment
at room temperature
REAGENTS & SUPPLIES:
•
•
•
•
•
•
Practice Gel Loading Solution
UltraSpec-Agarose™ powder
Concentrated electrophoresis buffer
1 ml pipet
100 ml graduated cylinder...
