Basic Tutorial of Flowjo

Flowjo

Data Analysis Software for Flow Cytometry

Version X User Documentation

Windows/Mac user documentation

Basic Tutorial

FlowJo was written by Adam Treister and Mario Roederer beginning in 1996, based on concepts developed at the

Herzenberg Laboratory at Stanford. We are indebted to our active and enthusiastic users worldwide for their ideas,

discussions and tireless testing of new versions.

FlowJo, its tutorials, documentation and web site are copyright © Tree Star, Inc. 1997-2013. All rights reserved.

• Basic Tutorial •

• © MMXIII •

Revision date: 16 April 2013

version 10.0.6

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Table of Contents

Introduction............................................................................................iii

Getting Started.......................................................................................1

Lesson 1 - The Workspace..................................................................3

Lesson 2 - Gating...................................................................................7

Lesson 3 - Statistics...............................................................................10

Lesson 4 - Batching in the Workspace...........................................12

Lesson 5 - Basic Table Editor Functionality...............................14

Lesson 6 - Basic Layout Editor Functionality............................18

Saving.........................................................................................................25

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Introduction - FlowJo Basic Tutorial

FlowJo is an integrated environment for viewing and analyzing flow cytometric data, presented in the form of a

Workspace. The Workspace contains a list of all of the data samples that you load, the gates, statistics and other

analyses that you apply, and the table and graphical layout that you design. The Workspace is saved as a FlowJo

document on your hard disk; when you reopen the document, you will see the status of your analysis as it...