Ethyl Glucuronide in Human Hair After Daily Consumption of 16 or 32 G of Ethanol for 3 Months

G Model

FSI-6344; No. of Pages 5

Forensic Science International xxx (2011) xxx–xxx

Contents lists available at ScienceDirect

Forensic Science International

journal homepage: www.elsevier.com/locate/forsciint

Ethyl glucuronide in human hair after daily consumption of 16 or 32 g of ethanol for 3 months§

¨ Robert Kronstrand a,b,*, Linda Brinkhagen a, Fredrik H. Nystrom c

a

Department of Forensic Genetics and Forensic Toxicology, National Board of Forensic Medicine, Artillerigatan 12, SE-587 58 Linkoping, Sweden Department of Medical and Health Sciences, Faculty of Health Sciences, Division for Drug Research, Linkoping University, SE-581 85 Linkoping, Sweden c Department of Medical and Health Sciences, Faculty of Health Sciences, Diabetes Research Center, University Hospital of Linkoping, SE-581 85 Linkoping, Sweden

b

A R T I C L E I N F O

A B S T R A C T

Article history: Available online xxx Keywords: Ethyl glucuronide Hair Controlled dosing LC–MS–MS

The overall objectives of the study were to develop a sensitive method for ethyl glucuronide (EtG) determination in hair and then investigate if a low or moderate intake of ethanol could be differentiated from total abstinence. Forty-four subjects were included in the study, 12 males (7 drinkers and 5 abstinent) and 32 females (14 drinkers and 18 abstinent). The study lasted 3 months and the female drinkers consumed one glass (16 g of ethanol) and the males consumed two glasses (32 g of ethanol) of wine (13.5–14%) daily. Hair samples were collected as close as possible above the skin and the proximal 2 cm were analyzed for EtG. Hair was cut into pieces of about 0.5 cm length and washed before incubation overnight in water and then extracted on Clean Screen EtG Carbon columns. The LC/MS/MS system consisted of a Waters ACQUITY UPLC connected to an API 4000 triple quadrupole instrument. Two transitions for EtG and one for the internal standard EtG-D5 were measured. The method was linear from...