Fatty Acid Determination Using Gas Chromatography

TITLE:

Optimization of flow rate and column temperature.

OBJECTIVE:

To optimize flow rate and column temperature to determine four type standard mixture of methyl which are methyl laurate (0.20 mg/ml), methyl myristate (0.20 mg/ml), methyl palmitate (1.0mg/ml) and methyl stearate (0.7 mg/ml).

ABSTRACT:

The factors which contribute to the best separation of mixture of methyl esters are examined. These factors included the affect of carrier gas flow rate on the isothermal and temperature programming GC separation of methyl esters. The elution rate of a compound depends on volatility of compound, column temperature, carrier gas flow rate and length of the column of the particular GC system. This experiment is examined gas chromatography, including the concepts of retention time and resolution using a mixture of methyl esters which were methyl2 laurate (0.20 mg/ml), methyl myristate (0.20 mg/ml), methyl palmitate (1.0mg/ml) and methyl stearate (0.7 mg/ml). At the end of this experiment the resolution is measured to identify how well species are separated.

INTRODUCTION:

About instrument

Gas chromatography is unique and versatile technique. In its initial stages of development it was applied to the analysis of gases and vapours from very volatile components. Chromatography is a common name for techniques based on the partition of the molecules to be analyzed between a mobile and a stationary phase. Separation is the result of different partitions of molecules between the two phases. Because the best separation of any solutes can be obtained under equilibrium conditions, analytical chemists prefer to use chromatographic systems that are as near to the equilibrium state as possible. Preparation chromatographic separations are generally not equilibrium processes. Efficient separation of compounds in GC is depend on the compounds travelling through the column at different rates. The rate at which a compound travels through a particular GC system depends on the...