Bioinformatic Using Diferent Technique

Submitted by: Submitted by

Views: 168

Words: 542

Pages: 3

Category: Science and Technology

Date Submitted: 11/21/2012 03:50 AM

Report This Essay

Summary feedback on key issues

Assessor:

Grade:

You have identified your enzyme and its properties correctly / partly correctly / incorrectly.

Your investigations and searches are complete / mostly complete / incomplete.

You have provided extensive / adequate / inadequate supporting information.

Your report demonstrates good / satisfactory / partial / inadequate understanding of the web site outputs.

Other comments:

Bioinformatics Report

Sequence file number: Seq09 Seq09 .txt

1. Results from BLASTn search (giving only one example of multiple matches to the same sequence from the same species/strain under different accessions, i,e whole genome, isolated gene, cDNA):

GenBank ID Description Score Frequency (E) value

XM_003314320.2

PREDICTED: Pan troglodytes phosphorylase 488 8e-135

XM_003314319.2

PREDICTED: Pan troglodytes phosphorylase 488 8e-135

XM_001145433.3

PREDICTED: Pan troglodytes phosphorylase 488 8e-135

XM_003831725.1

PREDICTED: Pan troglodytes phosphorylase 488 8e-135

XM_003314320.2 Homo sapiens phosphorylase, glycogen, liver 488 8e-135

2. Identity of gene & protein product, taken from best match above.

Protein name, species EMBL/Genbank code UniProt/PIR code

Glycogen phosphorylase (Liver), Homo sapiens NM_001163940.1

P06737

3. Physical properties:

pI: 5.31

Overall charge (i.e. +/- balance): Zero

GRAVY value: 0.907

Attach a print-out of a Kyte and Doolittle plot showing the hydrophobicity of your protein.

________________________________________

Using the scale Hphob. / Kyte & Doolittle, the individual values for the 20 amino acids are:

Ala: 1.800 Arg: -4.500 Asn: -3.500 Asp: -3.500 Cys: 2.500 Gln: -3.500

Glu: -3.500 Gly: -0.400 His: -3.200 Ile: 4.500 Leu: 3.800 Lys: -3.900

Met: 1.900 Phe: 2.800 Pro: -1.600 Ser: -0.800 Thr: -0.700 Trp: -0.900

Tyr: -1.300 Val: 4.200 : -3.500 : -3.500 : -0.490...