Submitted by: Submitted by ismail272ali
Views: 168
Words: 542
Pages: 3
Category: Science and Technology
Date Submitted: 11/21/2012 03:50 AM
Summary feedback on key issues
Assessor:
Grade:
You have identified your enzyme and its properties correctly / partly correctly / incorrectly.
Your investigations and searches are complete / mostly complete / incomplete.
You have provided extensive / adequate / inadequate supporting information.
Your report demonstrates good / satisfactory / partial / inadequate understanding of the web site outputs.
Other comments:
Bioinformatics Report
Sequence file number: Seq09 Seq09 .txt
1. Results from BLASTn search (giving only one example of multiple matches to the same sequence from the same species/strain under different accessions, i,e whole genome, isolated gene, cDNA):
GenBank ID Description Score Frequency (E) value
XM_003314320.2
PREDICTED: Pan troglodytes phosphorylase 488 8e-135
XM_003314319.2
PREDICTED: Pan troglodytes phosphorylase 488 8e-135
XM_001145433.3
PREDICTED: Pan troglodytes phosphorylase 488 8e-135
XM_003831725.1
PREDICTED: Pan troglodytes phosphorylase 488 8e-135
XM_003314320.2 Homo sapiens phosphorylase, glycogen, liver 488 8e-135
2. Identity of gene & protein product, taken from best match above.
Protein name, species EMBL/Genbank code UniProt/PIR code
Glycogen phosphorylase (Liver), Homo sapiens NM_001163940.1
P06737
3. Physical properties:
pI: 5.31
Overall charge (i.e. +/- balance): Zero
GRAVY value: 0.907
Attach a print-out of a Kyte and Doolittle plot showing the hydrophobicity of your protein.
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Using the scale Hphob. / Kyte & Doolittle, the individual values for the 20 amino acids are:
Ala: 1.800 Arg: -4.500 Asn: -3.500 Asp: -3.500 Cys: 2.500 Gln: -3.500
Glu: -3.500 Gly: -0.400 His: -3.200 Ile: 4.500 Leu: 3.800 Lys: -3.900
Met: 1.900 Phe: 2.800 Pro: -1.600 Ser: -0.800 Thr: -0.700 Trp: -0.900
Tyr: -1.300 Val: 4.200 : -3.500 : -3.500 : -0.490...