Enzymes

Assay ENZYME SUBSTRATE OTHER

vendor/cat amt +qnt vendor/cat amt + qnt vendor/cat

Achase none none none needed

Achase (h) Sigma/C-1682 0.1 mg X 1 none needed

Cox 1 Cayman/60100 10K X 8 Cayman/760112 10 vials Cayman/760114

Cox 2 Cayman/60120 10K X 8 N/A Cayman/760113

Plasma Esterase none none Cayman/760116

MMP 1 R+D/ 901-MP 10 µg X 12 Calbiochem/444221 1 mg X 3 Calbiochem/364205

MMP 2 Calbiochem/PF023 5 µg X 13 R+D/ ES001 1 mg X 4

MMP 3 Calbiochem/444217 5 µg X 13 Calbiochem/480450 1 mg X 1

MMP 7 R+D/907-MP 10 µg X 6 N/A N/A

MMP 9 Calbiochem/PF024 5 µg X 13 N/A N/A

MMP 13 Calbiochem/444287 5 µg X 13 N/A N/A

Caspase 1 ENZO/BML-SE168 5000 units X 2 ENZO/ALX-260-116 5 mg X 6

Caspase 2 ENZO/BML-SE175 5000 units X 2 N/A N/A

Caspase 3 ENZO/BML-SE169 5000 units X 2 N/A N/A

Caspase 4 ENZO/BML-SE176 5000 units X 2 N/A N/A

Caspase 5 ENZO/BML-SE171 5000 units X 2 N/A N/A

Caspase 8 ENZO/BML-SE172 5000 units X 2 N/A N/A

Caspase 10 ENZO/BML-SE174 5000 units X 2 N/A N/A

Beta Secretase Invitrogen/P2985 6 kits included in kit N/A Calbiochem/469000

Elastase Calbiochem/ 324681 2 X 50 µg none needed

CYP 1A2 (h) BD/456203 0.5nm X 1 N/A Sigma/G-6378

CYP 2A6 (h) BD/456254 0.5nm X 3 none needed

CYP 2B6 (h) BD/456255 0.5nm X 6 Molecular Probes/ E2282 1 X 5 mg

CYP 2C19 (h) BD/456259 0.5nm X 3 BD/451014 1 X 5 mg

CYP 2C6 (rat) BD/456536 0.5nm X 6 BD/451740 5 X 5 mg

CYP 2C9 (h) BD/456258 0.5nm X 16 N/A

CYP 2D6 (h) BD/456217 0.5nm X 13 None needed

CYP 2E1 (h) BD/456206 none needed N/A

CYP 2E1 (rat) BD/456521 none needed N/A

CYP 2J2 (h) BD/456264 0.5nm X 3 None needed

CYP 3A4 (h) BD/456202 0.5nm X 1 BD/451755 1 X 5 mg

CYP C19 (h) BD/456260 0.5nm X 3 None needed

CD45 Enzyme ENZO BML-SE135

VHR Enzyme ENZO BML-SE333

Also needed: NUNC/269620 4 cases 96 well clear plates

NUNC/237108 1 case 96 well black plates