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Date Submitted: 02/16/2013 11:39 AM
REVIEW
Vaccine Process Technology
Jessica O. Josefsberg,1 Barry Buckland2
1
BioEdge Consulting, LLC, 100 Jefferson Avenue, Miami Beach, Florida
Department of Biochemical Engineering, University College London, Torrington Place,
London, UK; telephone: 646-3692034; fax 305-675-2713; e-mail: buckland@biologicb.com
2
Received 2 December 2011; revision received 24 February 2012; accepted 27 February 2012
Published online 30 March 2012 in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/bit.24493
ABSTRACT: The evolution of vaccines (e.g., live attenuated,
recombinant) and vaccine production methods (e.g., in ovo,
cell culture) are intimately tied to each other. As vaccine
technology has advanced, the methods to produce the
vaccine have advanced and new vaccine opportunities
have been created. These technologies will continue to
evolve as we strive for safer and more immunogenic vaccines
and as our understanding of biology improves. The evolution of vaccine process technology has occurred in parallel to
the remarkable growth in the development of therapeutic
proteins as products; therefore, recent vaccine innovations
can leverage the progress made in the broader biotechnology
industry. Numerous important legacy vaccines are still in
use today despite their traditional manufacturing processes,
with further development focusing on improving stability
(e.g., novel excipients) and updating formulation (e.g.,
combination vaccines) and delivery methods (e.g., skin
patches). Modern vaccine development is currently exploiting a wide array of novel technologies to create safer and
more efficacious vaccines including: viral vectors produced
in animal cells, virus-like particles produced in yeast or
insect cells, polysaccharide conjugation to carrier proteins,
DNA plasmids produced in E. coli, and therapeutic cancer
vaccines created by in vitro activation of patient leukocytes.
Purification advances (e.g., membrane adsorption,...