Plant Physiology

LabPlant physiology summary

Result:

We used calcium chloride, abscisic acid, fusicocin on wheat leaf. There was no visible difference between calcium chloride and water solutions. In calcium chloride test tube, Stomata of wheat leaf were open same as water solutions. Absicis acid promotes stomata closure. So stomata of wheat leaf were closed. Fusicoccin made stomata of wheat leaf to open. Initial weight of water solution test tube was 11.21g and final weight was 10.99g. So 0.22g of water was lost in water test tube. Surface area of leaf that used in this test tube was 412 mm2 and WTLS was 5.34E-4. Initial weigh of abscisic acid test tube was 11.41g and final weight was 11.27g. Therefore, 0.14g of water was lost in this test tube. Surface area of leaf was 372 mm2 and WTLS was 3.76E-4. Initial weight of calcium chloride test tube was 9.63g and final weight was 9.31g. 0.32g of water was lost in this test tube. Surface area of leaf was 493mm2 and WTLS was 6.49E-4. Initial weight of fusicocin test tube was 11.33g and final weight was 11.05g. 0.28g of water was lost in this test tube. Surface area of leaf was 441mm2 and WTLS was 6.35E-4. Calcium chloride test tube had a largest amount of water lost and largest surface area of leaf. Percent open stomata of water, ABA acid, Calcium chloride, fusicocin was 40%, 0%, 30%, 80% respectively.

Discussion:

A) Yes, Fusicoccin makes stomata aperture size to be bigger and open. Abscisic acid makes stomata aperture to be closed. Water and calcium makes stomata aperture to be a little bit open. Basically, bigger size of stomata aperture causes more losing water. Because bigger size of stomata has more water that go outside of a leaf.

B) Upper surfaces of a leaf are more exposed to light. So there will be more evaporation and transpiration on upper surfaces. It means more water will be losing in upper surface of a leaf. Therefore, there are more stomata in lower surfaces of a leaf than upper surfaces.

C) More...