Catalase and Decomposition of Hydrogen Peroxide

NAME : KRISHNAVEYNI A/P KALIMUTHI

CLASS : DBT 4A

DATE SUBMITTED : 14 AUG 2015

EXPERIMENT 4: CATALASE AND THE DECOMPOSITION OF H2O2 (VIRTUAL)

PROCEDURE

1. Fill the beaker with water and 5ml of hydrogen peroxide.

2. Pipette 1ml of catalase enzyme into the beaker.

3. After 10 seconds, add sulfuric acid into the beaker.

4. Using titration method, check the presence of hydrogen peroxide in beaker.

5. Fill the burette with 5ml of potassium permanganate ( KMnO4).

6. Add KMnO4 drop by drop into the beaker until the H2O2 become purple color.

7. Record the data.

8. Repeat the step 1 until step 7 for 30, 60, 90, 120 and 180 seconds.

RESULTS

Determination of catalase activity

|Time (s) |10 |30 |60 |90 |120 |180 |

|Initial reading of KMnO4 (ml) | | | | | | |

| |5 |5 |5 |5 |5 |5 |

|Final reading of KMnO4 (ml) | | | | | | |

| |0.5 |1.5 |3 |4 |4.5 |4.9 |

|Amount of H2O2 that reacts with KMnO4 (ml)| | | | | | |

| |4.5 |3.5 |2 |1 |0.5 |0.1 |

|Amount of H2O2 that was broken down by | | | | | | |

|catalase |0.5 |1.5 |3 |4 |4.5 |4.9 |

|Amount of KMnO4 (ml) used | | | | | | |

| |4.5 |3.5...