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Date Submitted: 09/29/2013 03:23 AM
ANGIOGENIC PROPERTY OF GARCINIA MANGOSTANA LINN. AND SANDORICUM KOETJAPE MERR. IN CHORIOALLANTOIC
MEMBRANE OF QUAIL (COTURNIX COTURNIX JAPONICA)
EMBRYO
A Thesis Presented to the
College of Science Graduate Studies
De La Salle University-Dasmariñas
In Partial Fulfillment of the Requirements for the
Degree Master of Science in Biology
LAARNI E. GLORIANI
May 2012
ABSTRACT
Bioactive chemical compounds of plant origin may influence the angiogenic activity of blood vessels formation. The aim of this study was to investigate the effect of ethanolic extracts of S. koetjape and G. mangostana fruit hulls on the chorioallantoic membrane (CAM) angiogenic property on quail embryos. Extracts with concentrations of 10, 50, and 100µg/mL, having a total volume of 50 μl each, were applied on CAM surface of ex ovo prior to the transfer of shell-less cultures to the incubator. The study confirmed that exogenous application of both extracts at 10 μg/mL significantly (p0.05) less than the positive control.
With regard to the density of the branch vessel 10 µg/mL concentration of ex-ovo showed significantly lesser (p>0.05) density compared with the control groups and other treated groups. Aside from 10 µg/mL treatment, all the rest did not show significant difference in density. Hence, treatments 50 µg/mL and 100 µg/mL did not effectively lessen the density of the branch vessels.
The MVD of the capillary demonstrated that 10 µg/mL treated group did not show significant difference on the density of the vessels compared with the control group (SDS). However, the remaining treated groups did not show significant difference (p0.05) vary on the density regardless of the treatment concentration received at 10 µg/mL and 50 µg/mL . However, the capillary vessels significantly has higher (p