Scientists Determine the Function of Malondialdehyde

Malondialdehyde(MDA) is the organic compound with all the formula CH2. The framework with this species is more complex than this formula suggests. This reactive species occurs naturally and is a marker for oxidative stress. Malondialdehyde along with other thiobarbituric reactive substances condense with two equivalents of thiobarbituric acid to give a fluorescent red derivative whcih is assayed spectrophotometrically.

Malondialdehyde reacts with deoxyadenosine and deoxyguanosine in DNA, forming DNA adducts, the main one being M1G that is mutagenic. The guanidine band of arginine residues condense with malondialdehyde to present 2-aminopyrimidines. Malondialdehyde is reactive and potentially mutagenic. It has been seen in heated edible oils including sunflower and palm oils. g4f6yk9 Corneas of patients experiencing keratoconus and bullous keratopathy have raised degrees of malondialdehyde that based on one study. MDA is also used in tissue chapters from patients with osteoarthritis.

MDA ELISA Kit employs the competitive inhibition enzyme immunoassay technique. Antibody specific for MDA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase(HRP) conjugated MDA. A competitive inhibition reaction is launched between MDA (PRL ELISA Kit www.cusabio.com/ELISA_Kit-98148/) and HRP-conjugated MDA with the pre-coated antibody specific for MDA. The amount of MDA in samples, the less antibody bound by HRP-conjugated MDA. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of MDA in the sample. The color development is stopped and the intensity of the color is measured.