Spectrophotometer

Bachelor of Science (Hons) Biomedical Science

Bachelor of Science (Hons) Biomedical Science

Biological Science

BBS2112

Spectrophotometer and its function

Name : Joanne Wong

Student ID : 00000012636

Biological Science

BBS2112

Spectrophotometer and its function

Name : Joanne Wong

Student ID : 00000012636

Contents | Page |

Introduction | 3 |

Objective | 4 |

Materials | 4 |

Methods | 5 |

Results | 7 |

Discussion | 17 |

Conclusion | 19 |

Reference | 19 |

Name : Joanne Wong

Student ID : 00000012636 (BM1/14)

Title : Spectrophotometer and its function

Introduction

Spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength.[1] It can measure any of the listed light ranges that usually cover around 200 nm - 2500 nm using different controls and calibrations. [1] There are a few types of spectrophotometer such as calorimeter, UV spectrometer, IR spectrometer, atomic spectrometer and mass spectrometer. [2] It is being used widely in the field of physics, materials science, chemistry, biochemistry, and molecular biology[3] as it is an optical instrument that measure the amount of light absorbed by the solutes in the solution, thus measuring the absorbance of a solution. The mechanism behind this theory is based on the reference on the visible light spectrum. Different compounds absorb different wavelengths of light and appear to be the colour that it’s reflected which can be observed with our naked eyes. The higher the concentration of the absorbing compounds in a solution, the greater the amount of light that is absorbed.

The absorbance of a solution can be determined using the methods as follows:

1) Transmittance, T is the amount of light transmitted through a solution and it can be determined by using the formula of

A = log10 I0/I

A = 2 - log10 %T

Where I is the incident light

I0 is the energy of light transmitted through...