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Date Submitted: 11/17/2014 03:16 AM
BACHELOR IN SCIENCE PHARMACEUTICAL CHEMISTRY (HONS)
SEMESTER 1
BIOLOGICAL SCIENCE
PRACTICAL REPORT 1
TITLE: SPECTROPHOTOMETER AND ITS FUNCTION
LECTURER : DR.HO KET LI
NAME: SYARIFAH ANITH ADILA BINTI SYED ROZHAN
STUDENT ID:00000011352
COHORT :PC1/14
BENCH 2 , MDL 3.03.05
Table of content
Content | Page |
Introduction | |
Objectives | |
Materials | |
Part 1 : Determination of Amax of Bromophenol Blue * Method * Results * Table1.1 * Graph 1 * Discussion | |
Part 2 : The Effect of Concentration on Absorbance of Bromophenol Blue Solution * Method * Results * Table 1.2a * Graph 2 * Discussion | |
Part 3 : Determination of The Concentrations of The Bromophenol Blue Solutions of Unknown Concentration * Method * Calculation * Discussion | |
Part 4 : The Effect of Concentration on Absorbance of Methyl Orange Solutions * Method * Results * Table 1.2b * Graph 3 * Discussion | |
Part 5: Determination of The Concentrations of Two Different Solutes, Bromophenol Blue and Methyl Orange , in Mixture C * Method * Results * Table 1.3 * Table 1.4 * Calculation * Discussion | |
Conclusion | |
References | |
Introduction
The uses of spectrophotometer has been a huge contribution in finding the concentration of solute or compound in chemical and biological sciences [1]. It is an instrument where it measure the concentration of solute or compound by measuring the amount of light absorbed by a sample [2]. Every compound or solute can be determined using the wavelength from the spectrophotometer. Usually different compounds or solute can absorbed light at a different wavelength [1]. The sample that was measured is placed in a cuvette. Cuvette is a small square tube cell that is made of plastic, glass or an optical grade quartz that was designed to hold a sample [3]. The cuvette is a very delicate and it should be handle with care because it can affect the...