Bacllius, Escherichia, Mycoplasma

Diagnoses of an infected patient

Bacllius, Escherichia, Mycoplasma; are all microorganism but are very differnted. There are diffent ways to identify both straining and anatomically. I have been given a patient sputum sample and have been asked to identify which of this three genera is causing the infection.

Bacillus are Prokaryotic cells, that are rod shaped bacteria and can from long chains. Bacillus are also endospores(resting cells). While in this state, endospores are highly durable dehydrated cells with thick walls. Endospores can remain dormat for thousand of years and reture to its vegetative state be a proscess called germination. Germination is triggered by phyiscal or chemical damage to the endospore's coat. The endospore's enzymes then break down the extra layers sutrounding the endospore, water enters, and metabolism reumes. Bacillus are mostly gram-postive but some can be gram- negtive. Escherichia are rod shaped and gram-negative. Most of them are flagella motility (the abiltiy of an orgaism to move by itself). Mycoplasmas are the smallest known bacteria that can grow and reproduce outside living host cells. Due to their size they have no cell walls. When first identify they were mistaken for viruses, because pass through most bacterial filters. Their plasma membranes are unique amoung bacteria in having lipids called sterols, which are thought to help protect them from lysis (rupture). Mycoplasma is gram-positive when stained. Gram stain was developed in 1884 by the Danish bacteriologist named Hans Christian Gram. This procedure classifies bacteria into two large gram-positive and gram-negaitve. Gram-negitve bacteria flagella are anchored by two pairs rings. The outer pair of rings to various portions of the cell wall and the inner pair of rings are anchored to the plasma membrane. Gram-positive bacteria, only have a pair of inner rings. The flagellar protein called H antigen is used to distinguishing variation (serovars) within a species,of...