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Date Submitted: 04/28/2011 07:35 AM
Bacteriological changes
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The bacterial flora on live fish
Microorganisms are found on all the outer surfaces (skin and gills) and in the intestines of live and newly caught fish. The total number of organisms vary enormously and Liston (1980) states a normal range of 102-107 cfu (colony forming units)/cm2 on the skin surface. The gills and the intestines both contain between 103 and 109 cfu/g (Shewan, 1962).
The bacterial flora on newly-caught fish depends on the environment in which it is caught rather than on the fish species (Shewan, 1977). Fish caught in very cold, clean waters carry the lower numbers whereas fish caught in warm waters have slightly higher counts. Very high numbers, i.e., 107 cfu/cm2 are found on fish from polluted warm waters. Many different bacterial species can be found on the fish surfaces. The bacteria on temperate water fish are all classified according to their growth temperature range as either psychrotrophs or psychrophiles. Psychrotrophs (cold-tolerant) are bacteria capable of growth at 0°C but with optimum around 25°C. Psychrophiles (cold-loving) are bacteria with maximum growth temperature around 20°C and optimum temperature at 15°C (Morita, 1975). In warmer waters, higher numbers of mesophiles can be isolated. The microflora on temperate water fish is dominated by psychrotrophic Gram-negative rod-shaped bacteria belonging to the genera Pseudomonas, Moraxella, Acinetobacter, Shewanella and Flavobacterium. Members of the Vibrionaceae (Vibrio and Photobacterium) and the Aeromonadaceae (Aeromonas spp.) are also common aquatic bacteria and typical of the fish flora (Table 5.4). Gram-positive organisms as Bacillus, Micrococcus, Clostridium, Lactobacillus and coryneforms can also be found in varying proportions, but in general, Gram-negative bacteria dominate the microflora. Shewan (1977) concluded that Gram-positive Bacillus and Micrococcus dominate on fish from tropical waters. However, this...