Screening, Purification and Characterization of Extracellular Amyl

SCREENING, PURIFICATION AND

CHARACTERIZATION OF

EXTRACELLULAR AMYLASE FROM

BACTERIA

BY

ARPIT SHARMA(06BBT038)

SHOBHIT MALIK (06BBT210)

SUSHANT BHATIA(06BBT236)

(UNDER THE GUIDANCE OF DR. V. SUNEETHA)

CONTENTS:

1. Introduction

2. Methodology

3. Optimization of various growth parameters

4. Production of amylase

5.Extraction and partial purification

6.Effect of different metal ions on amylase production

7.Current Applications of Amylase

8. Future Prospects

9. References

INTRODUCTION:

Amylases refer to a group of enzymes whose catalytic

function is to hydrolyze (breakdown) sugar and starch.

Alpha Amylase:

•

Hydrolyze alpha-1, 4-glycosidic linkages, randomly yielding

dextrins, oligosaccharides and monosaccharides.

•

Endo- amylases.

•

Are calcium metalloenzymes

Beta Amylase:

• Exo-acting amylase

• Maltose--major end product.

• Catalyze the hydrolysis of second alpha- 1,4 glycosidic bond from nonreducing end

• cleaves off two glucose units (Maltose) at a time.

Gamma Amylase :

• Will cleave both alpha- (1,4) glycosidic linkages and alpha-(1,6)

glycosidic linkages.

• Most efficient in acidic environments.

CHARACTERISTICS OF BACILLUS

 Bacillus is a genus of endospore-forming, Gram positive rod-

shaped bacteria and a member of the division Firmicutes

 Sporulation of these cells is not repressed by exposure to air.

 They are Gram positive in early stages of growth and some are

also reported to be Gram negative.

 Cells of Bacillus may occur singly or in chains.

 The form of the endospore and the shape of the spore-bearing

mother cell, the sporangium is a characteristic feature of Bacillus

species.

METHODOLOGY

SAMPLE COLLECTION:

 Soil samples which had sandy texture were

collected from areas in and around rice mill

near Chittor bus stand, Katpadi.

 Samples contained paddy waste and thus were

expected to have amylase producing

microorganisms

SCREENING AND...