Biosciences

Student name: ………………………..…

(Please print)

Demonstrator………................................

Group…………………………………....

Faculty of Health and Medical Sciences

BMS1047

MOLECULAR BIOLOGY AND GENETICS

2015-2016

LABORATORY MANUAL

Module Organiser: Professor Andre Gerber

Academic Staff

Prof Graham Stewart

Dr Jenny Ritchie

Support Staff

Ian Wright

Demonstrators

Related web pages: Information about the practical will be found at https://surreylearn.surrey.ac.uk/

These will be updated as the module progresses.

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LIST OF CONTENTS

1. Introduction

2. Practical class assessment and submission deadlines

3. Health and Safety information

4. Tips on Laboratory Technique

5. The Experiment

5.1 Day 1

5.2 Day 2

5.3 Day 3

Page Number

2

2-3

3

4

5

7

9

13

Timetable of practical classes per group

Group

day 1

day 2

day 3

th

th

1

Tues 9 Feb

Friday 12 Feb

Tues 16th Feb

2

Friday 19th Feb

Tues 23rd Feb

Friday 26th Feb

3

Tues 1st March

Friday 4th March

Tues 8th March

4

Friday 11th March Tues 15th March Friday 18th March

All practical classes are held in 28/29AX01 at 2-5pm.

Overview of experiment and activities performed on each day

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1. Introduction

Welcome to the Molecular Genetics Practical Classes. The practical classes have been designed

to complement the lecture content of the module and provide you with hands-on experience of

techniques that help demonstrate essential concepts in molecular cloning and genetics.

Molecular cloning builds on our understanding of DNA as the ‘hereditary material’ or genetic

code of most organisms. In the practical class, you will perform experiments to demonstrate the

transfer of antibiotic resistance genes between bacterial cells by conjugation and monitor the

resultant changes in the organism using genotypic and phenotypic approaches. Changes in the

organism’s genotype will be assessed using polymerase chain reaction (PCR) and DNA sequence

analysis, whereas antibiotic...