Glycosis Full Lab Report

Abstract

This study examined glycolysis in a fermentation of Saccharomyces cerevisiae. This was tested by placing fermentation tubes of Saccharomyces cerevisiae solution in different temperature baths and measuring the accumulation of carbon dioxide in the tubes. The hypothesis was that at the hottest temperature, the fermentation rate would be the highest, and at the lowest temperature, the fermentation rate would be the lowest. There were four baths: a control, cold, warm, and hot bath. The tubes sat in the baths for a total of thirty minutes and were measured for their carbon dioxide was measured. After the tubes were measured, the data was recorded. From the recorded data the mean, the standard deviation, and the t-tests were calculated. The results proved the hypothesis correct. NEED MORE

Results

The average carbon dioxide for each of the levels of temperature was different. The highest average of carbon dioxide per minute is was the hot temperature with a rate 0.1744 milliliters of CO2/min. The lowest average of carbon dioxide was the low temperature. The t-test was 7.449 between the control and cold tubes, 1.94 between the control and warm tubes, and 4.26 between the control and hot tubes.

Discussion

The resulting data supported the hypothesis, which stated that the hottest temperature would be the best for fermentation. The experiment was performed by recording the carbon dioxide output of the yeast at different temperatures. The yeast that was in the hottest temperature had a carbon dioxide output of 0.1744 mL/min. The lowest carbon dioxide output was from the yeast in the cold temperature at 0.0344 mL/min. The low carbon dioxide production can be explained by Mair’s experiment (2005). At extremely cold temperature, the enzymes that are used during fermentation slow down and become unproductive in creating ATP. The fact that the highest temperature had the highest rate of fermentation does not correlate with Mair’s experiment. Mair’s experiment...