Antibacterial Activity of Premna Odorata Blanco (Alagaw)

Philippine Science High School Central Luzon Campus

ANTIBACTERIAL ACTIVITY OF Premna odorata Blanco (ALAGAW) LEAF EXTRACT ON Bacillus subtilis, Escherichia coli, AND Staphylococcus aureus

ANGELITA B. PANGILINAN MARCH 2013

Antibacterial activity of Premna odorata Blanco (Alagaw) Leaf Extract on Bacillus subtilis, Escherichia coli, and Staphylococcus aureus

by Angelita B. Pangilinan

ABSTRACT Alagaw (Premna odorataBlanco) is an important medicinal plant that possesses cardio tonic, antibiotic, anti-coagulant, carminative, hepatoprotective, and anti-tumor properties. It is proven to loosen phlegm and coughs. Literature showed that the genus Premna contains antimicrobial and antibacterial properties. The present study aims to evaluate the antibacterial activity of the leaves extract of P. odorata against the selected human pathogens (Bacillus subtilis, Escherichia coli, and Staphylococcus aureus). To do this, five concentrations of the extract were tested to the three bacterial species. The results revealed that the zones of inhibition produced by the extract are dose dependent because increased concentrations of the extract produce wider zones of inhibition. Based on the results, it can be concluded that P. odorata Blanco has antibacterial property.

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APPROVAL SHEET This is to certify that this research work entitled, “Antibacterial activity of Premna odorata Blanco (Alagaw) Leaf Extract on Bacillus subtilis, Escherichia coli, and Staphylococcus aureus”prepared and submitted by Angelita B. Pangilinan, to fulfill partial requirements in Science and Technology Research II was approved on ___ March 2013 .

___________________ RALPH S. HIPOLITO Research Adviser Science and Technology Research II Teacher

The Curriculum and Instruction Services Division endorses acceptance of this research work as partial fulfillment of the requirements in Science and Technology Research II. ___________________ ARIES N. OLIVEROS Chief, Curriculum and Instruction...