Determining Fe2+ and Fe3+ Concentration of Analyte in Aqueous Media Using Ceruloplasmin, Fe3+-Transporting Atpase, Nitrilotriacetic Acid in a Hepes/Tris Buffer

Determining Fe2+ and Fe3+ concentration of analyte in aqueous media using Ceruloplasmin, Fe3+-Transporting ATPase, nitrilotriacetic acid in a Hepes/Tris buffer

Vishal Kumar

kumarvish at g m a i l

Bradley University – Bachelors of Electrical Engineering

Northwestern University – Pre-Med Post Baccalaureate

Abstract : Ceruloplasmin functions to oxidize Fe2+ to Fe3+ in an acidic environment using six Cu2+ ion cofactors and ATP. This property is used with a Fe3+-Transporting ATPase to isolate Fe3+ ions across a membrane. The transported Fe3+ ions are reduced with oxygen in water. The resulting Fe2O3 is correlated with concentrations of Fe2+ and Fe3+ ions present in analyte.

Introduction: Iron is an abundant metal on the earth. It is most commonly found as Iron(II) oxide and Iron(III) oxide in the Fe2+ and Fe3+ oxidation state. Needless to say, it functions ubiquitously in biological processes, chemical processes, and is used extensively in manufacturing, and as building material. A low-cost approach is presented for the isolation and measurement of Fe2+ and Fe3+ ions in an analyte. Using the Ceruloplasmin enzyme and Fe3+-Transporting ATPase (FTA) enzyme, Fe3+ ions are transported and isolated across a membrane. The isolated Fe3+ ions are reduced with water to form Fe2O3(s). Analyte Fe2+ and Fe3+ ion concentration is determined from resulting Fe2O3(s).

Methods: The goal of the process is to measure the concentration of Fe2+ and Fe3+ ions of a given FexOx analyte solution. The analyte may be contaminated with unknown compounds. The entire process is performed in the reaction vessel. The reaction vessel consists of an oxidation chamber, an impermeable membrane embedded with FTA and a reduction chamber. The oxidation chamber consists of water, HCl(aq),CuCl2(aq), nitrilotriacetic acid (NTA), Ceruloplasmin, ATP, and Hepes/Tris buffer at pH 6.0. The acid facilitates the oxidization of added FexOx analyte into Fe2+ and Fe3+. Consequentially, FeCl2 (aq), FeCl3 (aq)...